Multiprotein Inhibitory Effect of Dietary Polyphenol Rutin from Whole Green Jackfruit Flour Targeting Different Stages of Diabetes Mellitus: Defining a Bio-Computational Stratagem

The anti-diabetic potential of whole unripe jackfruit (peel with pulp, flake, and seed) was investigated using inhibitory assays for α-glucosidase, α-amylase, aldose reductase, glycation at multiple stages. Using activity-guided repeated fractionation on a silica gel column chromatography, dietary flavonoid rutin potent antihyperglycemic activity extracted from the methanol extract flour (MJ). Rutin found to inhibit both α-glucosidase (IC50: 7.86 µg/mL) α-amylase 22.00 in competitive manner inhibition low Ki values. In addition, vitro experiments revealed that prevented each stage protein as well production intermediate molecules. Furthermore, significantly inhibited reductase 2.75 non-competitive manner. During silico studies, molecular docking dynamics simulation studies have suggested has high binding affinity enzymes studied, which could explain its effects. interacted key residues target enzymes’ inhibitor sites. Compared controls used, had higher efficiency stability pocket enzymes. According our findings, presence is more likely be associated MJ via action polyol pathway glycation. bio-computational study indicates lead all used an effective drug near future.